Analysis of thermophoresis for separation of polystyrene microparticles in microfluidic devices

We analysed the thermodiffusion phenomenon both numerically and experimentally for size-based separation of polystyrene microparticles. For model validation, we followed previously published numerical studies using ANSYS Fluent 2020 R2 software. our experimental analysis, defined a new microchannel geometry that would separate at least two groups particles (5 20 µm). trajectory microparticles in central channel microdevice under following conditions: without temperature gradient, with application thermal gradient parallel to gravitational field (cooling from bottom or top part), generation perpendicular direction gravity force. Numerical results these boundary conditions demonstrated that, terrestrial conditions, 5 µm larger microsized cannot be separated by thermophoresis flow because